Fig. 3

The validation of robustness of SpaDo. a Performance of SpaDo with different numbers of nearest neighbors on three single-cell spatial transcriptomic datasets. No. of NNs: number of nearest neighbors. b Performance of SpaDo with different radius. c Performance of SpaDo when using cell type annotation from original paper or Seurat v4 with different value of parameter “Resolution” on osmFISH, seqFISH, and STARmap datasets. R resolution. d Performance of SpaDo when using different spot deconvolution methods. e Performance of SpaDo when using different distance metrics on three single-cell spatial transcriptome datasets. f Performance of SpaDo when using 12 DLPFC datasets. g Performance of SpaDo with increased dropout rates on osmFISH dataset. The red line represents the original performance of each method. h Performance of SpaDo with increased dropout rates on DLPFC_151673 dataset. Percentage of extra dropouts is shown on the right of the plots. The red line represents the original performance of each method