Fig. 6

Dual-PWS reveals differential chromatin packing scaling and dynamics upon B-type lamin degradation. A Representative PWS images of HCT116^{LMN(B1&B2)−AID} cells before and after 24-h auxin treatment. Scale bar = 5 μm. Data are representative of three independent biological replicates (N = 3). B Violin plots for chromatin packing scaling (D) in HCT116^{LMN(B1&B2)−AID} cells before and after 24-h auxin treatment. C Violin plots for fractional moving mass in HCT116^{LMN(B1&B2)−AID} cells before and after 24-h auxin treatment. D Violin plots for diffusion coefficient in HCT116^{LMN(B1&B2)−AID} cells before and after 24-h auxin treatment. E Segmentation for PWS regional analysis. Region 1 is the nuclear periphery while region 7 is the nuclear interior. Scale bar = 5 μm. F Regional PWS measurements and percent changes of D in HCT116^{LMN(B1&B2)−AID} cells before and after 24-h auxin treatment. Error bars: SD. G Regional PWS measurements and percent changes of fractional moving mass in HCT116^{LMN(B1&B2)−AID} cells before and after 24-h auxin treatment. Error bars: SD. H Regional PWS measurements and percent changes of the diffusion coefficient in HCT116^{LMN(B1&B2)−AID} cells before and after 24-h auxin treatment. Error bars: SD. For B–H: Data was compiled from three independent biological replicates (N = 3). Significance was calculated by unpaired t test with Welch’s correction applied (**** < 0.0001). (Control n = 953; Auxin n = 869). The truncated violin plots in B–D extend from the minimum to the maximum value. The line in the middle of each plot is the median value of the distribution, and the lines above and below are the third and first quartiles, respectively. Each dot represents one cell