Fig. 3
Mesoscale chromatin structure is overall preserved upon B-type lamin degradation. A Representative normalized Hi-C trans-interaction matrices for chromosomes 1 and 15 in the control and 24-h auxin treatment conditions are shown for HCT116LMN(B1&B2)−AID cells. 15 kb resolution. B The eigenvectors for chromosomes 2 and 19 located at the nuclear periphery and interior, respectively, are shown. Pink: A compartment. Purple: B compartment. Eigenvectors computed by Juicer are the first eigenvector of the correlation matrix of the binned Hi-C contacts. C Contact probability scaling for HCT116LMN(B1&B2)−AID cells are shown for the control and 24-h auxin treatment conditions. Absolute values of s are indicated. D Raindrop plot comparing TAD sizes for the control and 24-h treatment conditions as revealed by TopDom. The number of TADs for each condition is indicated below the plot. Error bars: SEM. Significance was calculated by paired t test (**** < 0.0001). E Normalized Hi-C trans-interaction matrix demonstrating proximity of chromosome territories. The heatmap scale indicates the % change in interchromosomal contacts before and after 24-h auxin treatment in HCT116LMN(B1&B2)−AID cells. F Histogram demonstrating % change in interchromosomal contacts before and after 24-h auxin treatment in HCT116.LMN(B1&B2)−AID cells. G The Hi-C trans-interaction matrix demonstrates contacts across LAD segments, non-LAD segments and contacts across both segments for chromosome 5. The schematic under the matrix shows the partitioning of contacts across LAD segments and non-LAD segments for a representative chromosome. For A–G: Data was pooled from two independent biological replicates (N = 2)