Fig. 2

Loss of B-type lamins induces cell cycle arrest and altered nuclear morphology. A Propidium iodine staining in HCT116 and HCT116.^{LMN(B1&B2)−AID} cells to assess the percentages of cells in G1, S, and G2/M phase. Data are representative of three independent biological replicates (N = 3). B Flow cytometric analysis to measure relative DAPI-stained nuclear area from data collected with the ImageStreamX after 8 h of auxin treatment. Black: auxin-treated cells containing high mClover intensity. Green: auxin-treated cells with low or no mClover intensity. At least 20,000 events were recorded during the experiment. C Immunofluorescence shows the presence of nuclear blebbing in B-type lamin-deficient cells. Scale bar = 5 μm. Data are representative of three independent biological replicates (N = 3)