Fig. 7
From: Liquid–liquid phase separation of H3K27me3 reader BP1 regulates transcriptional repression
BP1ΔIDR2-C shows increased DON production and attenuated virulence in planta. A ChIP-qPCR analysis of relative H3K27me3 levels at TRI genes (TRI5, TRI4, TRI6, and TRI1) in wild-type PH-1, ΔBP1, BP1ΔIDR1-C, BP1ΔIDR2-C, and ΔBP1-C F. graminearum strains grown in toxin non-inducing conditions (YEPD) for 48 h. ACTIN served as a negative control. B Wild-type PH-1, ΔBP1, BP1ΔIDR1-C, BP1ΔIDR2-C, and ΔBP1-C strains were cultured in YEPD medium for 48 h. Total RNA was extracted for RT-qPCR analysis to determine relative transcript levels of TRI genes. Transcript levels were normalized to ACTIN. C DON production in the wild-type, ΔBP1 mutant, and complementation strains (BP1ΔIDR1-C, BP1ΔIDR2-C and ΔBP1-C) after 7 days of growth in YEPD medium. Different lowercase letters denote significant differences at P = 0.05 based on one-way ANOVA test. D, E Pathogenicity of the wild-type, mutant, and various complementation strains incubated in the central section spikelet of single flowering wheat head for 15 days (D) and on maize silks for 5 days (E)