Fig. 5

Pan-centromere analysis of the dynamic centromeric satellite repeats in Brachypodium genomes. a Sequence identity of merged fragments from WGS-seq reads to the CentBd or CentBs consensus sequence, as sampled in the pangenomes of B. distachyon. b PCA projection on PC1 and PC2 scores of sequencing fragments that cover satellite repeats from the pangenomes of different lines in the three Brachypodium species. The satellite fragments identified from the sequencing reads of different lines within the three Brachypodium genomes were merged, and 500 fragments were randomly selected for each genome. Box plots at the top and right of PCA projection show the overall distribution of PC1 and PC2 scores within each sub/genome group (T-test, ** P < 0.01). c Box plots show the overall distribution of PC1 scores among the individual line within the three Brachypodium species. ABR2 to Uni2 represents the lines from B. distachyon; ABR113 to Bhyb30 represents the lines from B. hybridum; ABR114 to TE4.3 represents the lines from B. stacei