Fig. 4

Ploidy prediction results for 4 methods (scAbsolute, HMMCopy, Ginkgo, and CHISEL) across single-cell DNA sequencing datasets from 3 different sequencing technologies (10X, DLP+, ACT) from cell lines and tumour samples. We only ran steps 1–3 of the scAbsolute algorithm. The data sets used are described in detail in the “Methods” section. Experimental ploidy annotation for the 10X data was based on karyotype information, and for the ACT data was based on FACS sorted cells using DAPI staining. Grey ranges of \(\pm 0.5\) around the experimental point estimate (red cross) of the sample ploidy are indicated. Blue asterisks indicate ploidy levels of 1/2 or 2 times the experimental ploidy estimate. For DLP+ data, no experimental ploidy annotation was available, but estimates were in accordance with ploidy estimates for the respective cell lines based on public karyotype information