Fig. 3

Detection of replicating cells across different sequencing technologies and cell lines. a Distribution of cycling activity in samples sequenced with three different sequencing technologies: 10X, ACT, and DLP+. Uniform and varying CN state indicates whether the copy number calls indicate a mostly diploid genome (as in G1/G2) or cycling cells (non-uniform CN state), respectively. b Distribution of cycling activity for G1 (solid line) and S phase cells (dotted line). The median of the distribution (blue line) is estimated and the left side of the distribution is used to determine a standard deviation that covers the majority of cells in G1 phase (red lines) and to determine an appropriate cutoff value to identify cells in S phase. c Cycling activity for normal, diploid cells (A73044A and A90553C), and a cancer cell line (T-47D) sequenced with DLP+ technology. The cell cycle annotation is based on sorting cells with FACS using DAPI staining and grouping cells either into apoptosis state (D) or in the three cell cycle stages (G1, S phase, G2). This measure can only be reliable estimated for normal, diploid cells and is not applicable in other samples. d Cycling activity for PEO1 FUCCI cells that have been sorted into different cell cycle stages based on the FUCCI marker system. The cells have been sequenced with a modified version of the DLP+ protocol (mDLP+)