Fig. 3
HI paternal chromosomes decompact when subjected to the N2 maternal environment. A Example of Z-projections of raw data collected during haploid-specific chromosome tracing and schematic representation of image segmentation, tracing and sorting of traces. Scale bar, 5µm. B ChrV mean distance matrix for traces derived from hybrid embryos (2–40cells) from crosses between N2 hermaphrodites and HI males, N2 maternal traces (left) and HI paternal traces (right), colored by distance (in μm). N = 1384 (N2m) & N = 1066 (HIp). Axis numbering represents positions across the chromosome as shown in Figure 1C. C Power-law fits of mean pairwise distance for N2m data (magenta) and HIp data (green). s = scaling exponent of spatial distance, a = step size. Values in brackets are 95% confidence intervals of the fit. P-values are acquired from a linear regression analysis of log-transformed data. D Normalized mean spatial distances for N2m and HIp traces shown as observed over expected spatial distances as determined by the power-law fit in C. Red denotes regions that are closer together than expected from the fit and blue regions that are further away. E Differences in normalized mean spatial distances between N2m and HIp traces (left) and p-value (right). Red denotes regions that are closer together than expected from the fit and blue regions that are further away. F Power-law fits of mean pairwise distance for N2m data (magenta) and N2 homozygous data (black). s = scaling exponent of spatial distance, a = step size. Values in brackets are 95% confidence intervals of the fit. P-values are acquired from a linear regression analysis of log-transformed data. G Power-law fits of mean pairwise distance for HIp data (green) and HI homozygous data (black). s = scaling exponent of spatial distance, a = step size. Values in brackets are 95% confidence intervals of the fit. P-values are acquired from a linear regression analysis of log-transformed data. H Differences of mean pairwise distances in µm (left), and p-value (right) of N2m and N2 homozygous (top) and HIp and HI homozygous (bottom). Red marks regions that are closer together than expected from the fit and blue regions that are further away. Axis numbering in B, D, E, and H represents positions across the chromosome as shown in Fig. 1C for all panels