Fig. 2

Highly similar N2 and HI ChrV organization. A Power-law fits of mean pairwise distances in µm versus genomic distance in Megabases (Mb) for N2 data generated in this study (black) and previously (red) [26]. s = scaling exponent of spatial distance, a = step size. Values in brackets are 95% confidence intervals of the fit. P-values are acquired from a linear regression analysis of log-transformed data. B ChrV mean distance matrix for N2 (left) and HI (right) in early embryos (2–40 cells), colored by distance (in μm). N = 6072 (N2) & N = 5905 (HI). C Power-law fits of mean pairwise distance for N2 data (magenta) and HI data (green). s = scaling exponent of spatial distance, a = step size. Values in brackets are 95% confidence intervals of the fit. P-values are acquired from a linear regression analysis of log-transformed data. D Normalized spatial distances for N2 date (left) and HI data (right). Data is plotted as observed over expected. The expected spatial distance is determined by the fit to the power-law function shown in C. Red marks regions that are closer together than expected from the fit and blue regions that are further away. The right panel shows the significant mean changes. E Changes between normalized spatial distances of N2 and HI data (left) and p-value of the spatial distance changes. Red marks regions that are closer together than expected from the fit and blue regions that are further away. F Mean pairwise distances in µm of subpopulations of chromosome conformations for N2 and HI traces as determined by unsupervised clustering. N2 and HI traces were pooled as outlined in H. G Distribution of traces within clusters from F shows both strains have similar cluster frequencies. Chi-square statistic was 29.94, p-value = 5.03 × 10⁻⁶ and Cramér’s V = 0.08. H Strategy for co-clustering to determine statistical similarity. Axis numbering in B–F represents positions across the chromosome as shown in Figure 1C