Fig. 5

SLAM-seq reveals hundreds of zygotically expressed transcripts post-transcriptionally regulated by miR-430. A Schematic representation of finding maternal-zygotic miR-430 targets whose decay is masked by de novo transcription. Maternal-zygotic genes (excluding known miR-430 targets) with non-significant decay at the total mRNA level between 2 and 7 h post-fertilization (foldchanges > −1.5) but with a significant decrease (adjusted p-values ≤ 0.05, fold change < 0) in total mRNA vs unlabeled mRNA at 7 h were selected. B Cumulative distributions of total vs unlabeled mRNA at 7h (B, left), Control LNA vs miR-430-LNA unlabeled (maternal, B, middle) and labeled (zygotic, B, right) mRNA Log2(fold changes), genes whose decay is masked by de novo transcription that contain a miR-430 target site in their 3′UTR (violet) or without miR-430 target site (black). Number of genes in each category is shown, along with p-values from Kolmogorov-Smirnov pairwise comparison tests. C Pie chart highlighting the genes used in the analysis. Cumulative distributions of Control LNA vs miR-430-LNA labeled (zygotic, C, middle) mRNA Log2(fold changes), for pure zygotic putative miR-430 targets (blue) and non-targets (black). Number of genes in each category is shown, along with p-values from Kolmogorov-Smirnov pairwise comparison tests. D Schematic representation of main findings from this figure. miR-430 post-transcriptionally regulates the zygotic mRNAs. During ZGA newly expressed mRNA targets of miR-430 show less stability than those that do not