Fig. 4

SLAM-seq reveals that known microRNA-430 regulates zygotic mRNA of its targets. A Schematic representation of experimental setup of SLAM-seq in LNA-injected embryos. Embryos injected with s4-UTP were either co-injected with miR-430-LNA or Control LNA, then collected at ~6h post-injection in six replicas. While miR-430-LNA reduces miR-430 function by competing with targets, control LNA does not affect miR-430 function. B Cumulative distributions of total (right side); unlabeled (maternal, middle) and labeled (zygotic, left) mRNA Log2(fold changes) between Control LNA and miR-430-LNA, highlighting previously determined miR-430 non-target controls (black), and miR-430 targets (known targets, red, [28]) and all other genes (gray). Number of genes in each category is shown, along with p-values from one-sided Kolmogorov-Smirnov pairwise comparison tests. C Schematic representation of main findings from this figure. Decreasing miR-430 function (miR-430-LNA) leads to increment in labeled reads of miR-430 targets when compared to control LNA. Thus, besides pure maternal mRNA clearance, miR-430 post-transcriptionally regulates the zygotic fraction of its targets. This regulation is alleviated when the microRNA function is decreased