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Fig. 5 | Genome Biology

Fig. 5

From: Precise fine-turning of GhTFL1 by base editing tools defines ideal cotton plant architecture

Fig. 5

Phenotypic characterization of WT and T1 progenies from GhTFL1L86P and GhTFL1K53G+S78G mutants. a Representative images of WT and T1 progenies of GhTFL1L86P at different growth stages. GhTFL1L86P plants show the cluster terminal flowers at the top and all axillary meristems subsequently produce floral buds directly on the main stem. b Representative images of WT and T1 progenies of GhTFL1K53G+S78G. The plants show semi-dwarf height, terminating clusters of flowers forming at the tips of the main stems and fruiting branches as monotypic fruiting branches, terminating sympodial growth. c, d Plant heights (c) and days to bud stage (d) of WT and the T1 progenies of GhTFL1L86P and GhTFL1K53G+S78G. All data are presented as mean ± sd. *P < 0.05, **P < 0.01, ***P < 0.001 and ****P < 0.0001 by two-tailed Studentʼs t-test. e The genotype analysis of the T1 progenies of GhTFL1L86P and GhTFL1K53G+S78G. The ordinate represents the target site information, including the 20 bp sgRNA sequence and the adjacent 3 bp PAM sequence, for the T1 generation individual plants of GhTFL1L86P and GhTFL1K53G+S78G. Each dot represents the editing efficiency of an independent sample

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