Fig. 4
From: Precise fine-turning of GhTFL1 by base editing tools defines ideal cotton plant architecture
The artificial evolution of GhTFL1 mediated by base editing generates novel plant architecture cotton mutants. a Mutants of the GhABE8e-induced L86P amino acid substitution in the CDS of the GhTFL1 gene exhibit an early flowering, compact inflorescence phenotype. Scale bar, 5 cm. GhTFL1L86P mutants develop rare twin flowers. b The T-to-C base editing led to amino acid substitution from leucine to proline at amino acids position 86 of GhTFL1 and the editing efficiency is analyzed by CRISPResso2 for quantification. Three plants tested carried two types of mutations and were chimeric. c The T0 seedlings carrying the K53G and S78G mutations had a double ball phenotype. Scale bar, 5 cm. d The A-to-G base editing led to amino acid substitution from Lysine to Glutamicacid/Glycine and Serine to Glycine at amino acids position 53 and 78 of GhTFL1 and the editing efficiency is analyzed by CRISPResso2 for quantification. This plant tested carried two types of mutations and was chimeric. e The plants with base editing of GhTFL1 promoter at -783 bp showed that leaves become more numerous and smaller, with darker leaves and more nutritional growth, and the editing efficiency is analyzed by CRISPResso2 for quantification. Scale bar, 5 cm. Two plants tested carried two types of mutations and were chimeric. f The plants with base editing of GhTFL1 promoter at -485-504 bp showed excessive nutritional growth and the editing efficiency is analyzed by CRISPResso2 for quantification. Scale bar, 5 cm. This plant tested carried two types of mutations and was chimeric