Fig. 2

Genome-wide analysis of DNA and RNA off-target effect for the GhABE8e system by whole genome sequencing and whole transcriptome sequencing. a Sequence alignment of sgRNA13 on cotton genome by IGV browser views. The A-to-G mutations edited by GhABE8e were detected at the A5, A6, and A8 of target region. The target sgRNA sequences are highlighted in different colors. b Numbers of total SNVs identified in the GhABE8e, GhABE8e without sgRNA, WT, and negative plants. c Distribution characteristics of DNA and RNA off-target SNVs (A-to-G/T-to-C) on cotton chromosomes in GhABE8e without sgRNA and GhABE8e edited plants. d UpSet diagram of off-target SNVs (A-to-G/T-to-C) at DNA and RNA levels in GhABE8e without sgRNA and GhABE8e edited plants. e Venn diagrams of DNA-SNVs and Cas-OFFinder predicted off-target sites for edited lines. f Venn diagrams of RNA-SNVs and Cas-OFFinder predicted off-target sites for edited lines. g The number of different locations of SNVs for GhABE8e without sgRNA and GhABE8e-edited T0 plants at DNA and RNA levels