Fig. 2
From: Undetectable off-target effects induced by FokI catalytic domain in mouse embryos
Undetectable off-target effect induced by homodimer-FokI-based fRYdCas9 in mouse embryos. A Schematic of homodimer fRYdCas9. B On-target efficiency of Cas9, RYCas9, fCas9, and fRYdCas9 for genes including AAVS1, EMX1, and VEGFA in HEK293T cells. C On-target efficiency of tdTomato+ and tdTomato− cell for the treated groups including Cre, Fokl-Homo, RYCas9, fRYdCas9, and fRYdCas9-Tyr in mouse embryos. D, E Comparison of the total number of detected Indels (D) and SNVs (E) for all groups. F, G Overlap among Indels (F) and SNVs (G) of fRYdCas9-Tyr-L+R detected by GOTI with predicted off-targets by Casoffinder. All P values were calculated by two-sided Student’s t-tests. n ≥ 3 replicates were used in all experiments.Two Cre samples were derived from Zuo et al. [8]