Fig. 1
From: Undetectable off-target effects induced by FokI catalytic domain in mouse embryos
Undetectable off-target effect induced by heterodimer-FokI-based TALENs in mouse embryos. A Analyzed the DNA off-target effects of FokI-based genome editing tools using GOTI. B On-target efficiency of tdTomato+ and tdTomato− cell on the basis of WGS for the treated groups including Cre, Rosa26-ELD-KKR, Rosa26-ELD, Rosa26-KKR, MSTN-ELD-KKR, MSTN-ELD, MSTN-KKR, NLS-ELD-NLS-KKR, NLS-ELD, and NLS-KKR. C, D. Comparison of the total number of detected Indels (C) and SNVs (D) for the examined groups. E, F Overlap among Indels (E) and SNVs (F) of Rosa26-TALENs detected by GOTI under predicted off-targets by TALENoffer. All P values were calculated by two-sided Student’s t-tests. n ≥ 3 replicates were used in all experiments. Two Cre samples were derived from our previous study [8], while an additional Cre sample was newly derived in the present study