Fig. 4
3′ UTRs’ RSS is inversely related to transcript accumulation in Arabidopsis, rice, and human. a Schematic of DIM-2P-seq used for in vivo probing 3′ end RSS of polyadenylated transcripts. See “Methods” for details. b DMS reactivity profile of stop codon regions (lower panel, CDS, 100 nt upstream of the stop codon, and 3′ UTR, 100 nt downstream of the stop codon). mRNAs were aligned by their stop codons (vertical red lines). 6996 genes were used in this analysis. P value = 5.993e−32 by Wilcoxon test between DMS reactivities of CDS and 3′ UTR. Nucleotide frequency around the stop codon regions was also shown (upper panel). c, e, f Comparison of gene expression level (RPKM) between the high-Gini and low-Gini genes for Arabidopsis (c), rice (e), and human (f). P value < 2.2e−16 by Wilcoxon test. Horizontal lines in the boxplots display the 75th, 50th, and 25th percentiles, respectively. The upper fence is 75th percentile + 1.5 * interquartile range. The lower fence is 25th percentile − 1.5 * interquartile range. Dots represent the outliers. d Biological processes of GO analysis for the high-Gini and low-Gini genes. P value cutoff, 0.01