Fig. 6

Changes in m6A levels between species are associated with coherent changes in gene expression levels. a The fold-change of gene methylation levels (m6A gene index) between S. cerevisiae and S. paradoxus alleles in the ndt80Δ/Δ hybrid (y-axis) depicted as a function of the fold-change in gene expression levels (measured as sample TPM) between the alleles appears at the x-axis. Higher methylation levels of an allele correlate negatively with its expression in ime4 WT (left) but not in a methylation-lacking (ime4Δ/Δ) strain, indicating abolishment of the association. b Quantification of m6A levels via m6A-sample index along a dense meiotic time course. Values for the ndt80Δ/Δ mutant and for ndt80Δ/Δ ime4Δ/Δ double mutants are displayed as positive and negative controls, respectively. c Calculation of Pearson R for agreement between differences in methylation and in expression across the two alleles (as in panels a and b), at different time points after induction of yeast meiosis in the WT hybrid strain. The anticorrelation between allele-specific methylation and allele-specific expression increases dynamically with the gaining of methylation during meiosis progression up to prophase, and again continuously lost with the decrease in methylation following prophase