Fig. 4

A Distribution of the target-level adjusted p-values (FDR) for all significant element-to-gene (E2G) pairs detected, split by how many gRNAs have raw gRNA-level p-values < 0.05. E2G pairs supported by three or four gRNAs are high confidence (dark blue), E2G pairs supported by 2 gRNAs are medium confidence (blue), and E2G pairs supported by a single gRNA are low confidence (light blue) and were discarded from downstream analyses. B Barplot indicating the number of high and medium confidence significant differentially expressed genes (DEGs) detected for non-coding perturbations within 1 Mb up/downstream of the target site. Targets from Gasperini enhancers are shown in red; ENCODE cCREs are shown in orange if they lie within a gene intron and in yellow if they are intergenic. C Density plot of the distance between the E2G pairs, in kilobases. DEGs from targets of different classes are shown separately, as indicated by the same colors used in B. D Boxplots of the distance between E2G pairs (as in C) but split by whether the gene is the nearest expressed gene to the target. The median is indicated.