Fig. 1

LOY detection by single nucleus multiome sequencing. A UMAP of male and female kidney cell types. PCT, proximal convoluted tubule; PST, proximal straight tubule; PT_VCAM1, VCAM1 + proximal tubule; PEC, parietal epithelial cells; TAL1, cortical thick ascending limb; TAL2, medullary thick ascending limb; DCT1, early distal convoluted tubule; DCT2, late distal convoluted tubule; PC, principal cells; ICA, type A intercalated cells; ICB, type B intercalated cells; PODO, podocytes; ENDO, endothelial cells; FIB_VSMC_MC, fibroblasts; vascular smooth muscle; and mesangial cells; TCELL, T cells; BCELL, B cells; MONO, mononuclear cells. B ATAC modality LOY density plot in 23,333 male cells. C RNA modality LOY density plot in 23,333 male cells D Joint ATAC/RNA LOY density plot in 23,333 male cells. E Median proportion LOY in male kidney cell types. F Gene ontology pathway enrichment for age-adjusted differentially expressed genes associated with LOY in the male proximal tubule