Fig. 6
Metagenomic and metatranscriptomic analysis of the rumen microbiome from the LME and HME sheep. A The bar plot shows methane (CH4) yields and the total gene expression of rumen Methanomassiliicoccales communities in the LME and HME sheep. Differences in gene expression between the two groups are compared. B A heatmap displaying the genomic expression of 10 Methanomassiliicoccales and four Methanobrevibacter genomes from G. 16, G. 21, and G. 22 significantly differing in the LME and HME sheep. The expression of these genomes between the two groups was standardized using the Z-score method, and an average value was calculated. C A phylogenetic tree was constructed using Methanomassiliicoccales MAGs assembled from the LME and HME sheep and other Methanomassiliicoccales genomes. These four MAGs are presented in red font, and their respective sample sources are labeled. D Boxplots display the total gene expression of seven genes (btuF, btuC, btuD, cbiM, cbiN, cbiQ, and cbiO) related to the microbial B12 and cobalt transport systems in the LME and HME sheep. Schematics of the microbial cobalt transport system are also included. The significance level between the two groups has been compared. Wilcoxon rank-sum test, ***P < 0.001. DMI, dry matter intake; TPM, transcripts per million