Fig. 4

Overview of metabolic potentials in Methanomassiliicoccales. The detected pathways and genes in the 243 Methanomassiliicoccales genomes related to glycolysis, the rTCA cycle, amino acid biosynthesis, methanogenesis, cofactor, and vitamin biosynthesis, Wood-Ljungdahl pathway, and various transporters. Different background colors represent different metabolic modules. The rays emitted by vitamin B₁₂ represent the genes encoding cobamide-dependent functions. Colored circles and gray lines indicate the frequency of the genes present in the 243 genomes. Detailed gene information in each pathway is presented in Additional file 8: Table S7. GAP, glyceraldehyde-3-phosphate; PEP, phosphoenolpyruvate; MF, methanofuran; H₄MPT, tetrahydromethanopterin; CoA, coenzyme A; CoB, coenzyme B; CoM, coenzyme M; DMS, dimethylsulfide; Fd, ferredoxin; ALA, δ-aminolevulinate