Fig. 8

A screenshot of an app that was used as a GUI to perform manual inspection and classification of LAMP testing for SARS-CoV-2 viral RNA [32, 33]. The app was used during our SARS-CoV-2 surveillance study [34] and for voluntary testing for COVID-19 infection on campus (University of Heidelberg) in 2020/2021. To the right, the app shows a 96-well plate layout colored either by content type (sample, empty, positive or negative control) or by the assigned result. To the left, it shows the results of three tests and one control for each sample. Accumulation of the LAMP product is indicated by the change of color from red to yellow and is measured as a difference in absorbance on two wave lengths. This difference is plotted as a function of time. Besides exploration (highlighting the corresponding lines for each sample), the app allows to manually reassign status, store results, and send them to the server, where they can be queried by the test subjects. The app is provided as an R script; the code and some example data are available on GitHub at https://github.com/anders-biostat/lamp_plate_analysis