Fig. 6

AR agonist-induced shared AR and GATA3 target genes are implicated in development and differentiation of mammary epithelium. A RNA-seq heatmap of differentially expressed (FDR < 0.05) mammary lineage marker genes associated with a DHT-induced shared AR and GATA3 binding event in T-47D and B MDA-MB-453 cells. Luminal marker genes are denoted by pink squares (y-axis) and basal marker genes are denoted by blue squares. Gene expression is represented as the log counts per million (logCPM) so as to observe expression differences between genes (rows). Genes common to all ER+ (T-47D, ZR-75-1) and ER- (MDA-MB-453, MFM-223) cell lines, as shown in C, are indicated in purple text. D Example genome browser images showing averaged GATA3, AR and H3K27ac ChIP-seq signals at binding sites associated with KDM4B (left panel), EHF (middle panel), and CNTNAP2 (right panel) in T-47D, E MDA-MB-453 cells, and F GAR15-13D ER+ PDX. Data represents the consensus signal of three independent replicates. G KDM4B, EHF, and CBTBAP2 transcript expression (logCPM) in GAR15-13D ER+ PDX tumors treated with DHT or enobosarm from [8]. Data was analyzed by ordinary two-way ANOVA followed by Dunnett’s multiple comparisons test comparing DHT or enobosarm treatment to vehicle (Veh) for each gene, where P < 0.05 versus Veh