Fig. 1

GATA3 is a novel AR interacting protein in breast cancer. A Up-set plot representing common and unique AR interacting proteins identified in ER+ and ER- breast cancer cell lines. B Classification of the 11 AR interacting proteins common to ER+ and ER- in vitro breast cancer models based on their molecular functions. C Multi-labeled immunofluorescence for AR (red) and GATA3 (green) in T-47D cells with nuclear (Dapi; blue) and cytoskeletal (F-actin; orange) labels. Scale bar = 30 μm. D, E Proximity ligation assays (PLA) showing AR-GATA3 interactions in D T-47D (ER+) and E MDA-MB-453 (ER-) breast cancer cell lines treated with Vehicle (Veh; EtOH) or the AR agonist 5-α-dihydrotestosterone (DHT; 10 nM). Scale bar = 15 μm. Quantification represented as mean foci per 300 nuclei ± SEM (n = 5 technical replicates) analyzed by one-sided unpaired t-test (***P < 0.001; ****P < 0.0001). F Western blot showing co-immunoprecipitation of AR and GATA3 in ER+ (top panel) and ER- (bottom panel) breast cancer cell lines treated with Veh (EtOH) or DHT (1 nM). G PLA for AR and GATA3 interaction in non-malignant and malignant (ER+ and ER-) clinical breast tissues. Scale bar = 30 μm