Fig. 2

Dual-specific splice sites (DSSs) are used as both 5′ss and 3′ss and are underrepresented in annotated splice sites. a The distribution of 5′ss (left) and 3′ss (right) MaxEnt scores for splice sites with an AGGT motif that function as either DSSs or single splice sites from our MPRA (Mann–Whitney test). b The distribution of 5′ss (left) and 3′ss (right) MaxEnt scores for splice sites with an AGGT motif that function as either DSSs or single splice sites from SRA splice junction data (Mann–Whitney test). c The 5′ss (left) and 3′ss (right) motifs of single splice sites with an AGGT motif (top) compared to DSSs (bottom) from the SRA data. d The percentage of annotated 5′ss and 3′ss compared to all 5′ss and 3′ss in the SRA junction reads (minimal threshold > 10 reads) which exhibited DSS activity (> 10 reads supporting 5′ss usage and > 10 supporting 3′ss usage). p-values were calculated from the chi-square test of independence