Fig. 3

FMRP-dependent localization and translation of NMD and FMRP targets in the axon relative to soma of mouse retinal ganglion cells. a Box and whisker plots as in Fig. 2b using mRNAs defined in Fig. 2a, after removing outliers, and data deriving from retinal ganglion cell axon and soma of Fmr1-KO mice relative to WT mice [14]. P values were calculated as in Fig. 2b. b Cumulative fraction of log₂ fold-change RNA enrichment (axons/somata) using mRNAs defined in Fig. 2a, and data deriving from retinal ganglion cell axon and soma of Fmr1-KO mice relative to WT mice [14]. c Box and whisker plots as in Fig. 2b using mRNAs defined in Fig. 2a, after removing outliers, and data deriving from retinal ganglion cell axons of Fmr1-KO mice relative to WT mice [14]. P values were calculated as in Fig. 2b. d Cumulative fraction of log₂ fold-change axonal RNA enrichment using mRNAs defined in Fig. 2a, and data deriving from retinal ganglion cell axons of Fmr1-KO mice relative to WT mice [14]. e As in c, but using data deriving from retinal ganglion cell somata of Fmr1-KO mice relative to WT mice [14]. P values were calculated as in Fig. 2b. f As in d, but using data deriving from retinal ganglion cell somata of Fmr1-KO mice relative to WT mice [14]. g As in a, box and whisker plots showing log₂ fold-changes in axonal translation (Axon-TRAP-seq normalized to mRNA abundance determined using EU-seq) for mRNAs defined in Fig. 2a, after removing outliers, and data deriving from retinal ganglion cell axons of Fmr1-KO mice relative to WT mice [14]. P values were calculated as in Fig. 2b. h Cumulative fraction of log₂ fold-change in axonal translation, (Axon-TRAP-seq normalized by EU-seq) using mRNAs defined in Fig. 2a, and data deriving from retinal ganglion cell axons of Fmr1-KO mice relative to WT mice [14]