Fig. 1
From: Compact CRISPR genetic screens enabled by improved guide RNA library cloning
Factors affecting guide cloning uniformity. A Violin plots depicting guide abundance distributions of libraries prepared with three different polymerases (Klenow, Klenow exo-, and NEB Q5 Ultra II) and inserts extracted at 70˚C. B Violin plots of libraries prepared the three different polymerases and a 37˚C extraction. C Melting temperature (Tm) and abundance of the lowest, mid, and top 20 guides in the 752-element pilot library. D Mann–Whitney U test comparing Tms of high (top 5%) and low (bottom 5%) abundance guides in the 752-element pilot library. E Correlation between forward and reverse complement oligo pools for CRISPRi V2 (top) and CRISPRa V2 (bottom) using a linear least-squares regression. F Number of guides missing from the oligos pools in forward, reverse, or combined sets in the CRISPRi V2 and CRISPRa V2 guide libraries. G Mann–Whitney U-test comparing the Tms of high (top 5%) and low (bottom 5%) abundance guides in the CRISPRi V2 genome-wide library