Fig. 6

Evaluating variant calling accuracy from RNA and protein data by benchmark variants constructed from DNA data. a Schematic of central dogma and the number of variants detected in the Quartet DNA-seq, RNA-seq, and LC-MS/MS-based proteomics datasets. b Validation of Quartet RNA variants using DNA reference datasets. True positive (TP) means RNA variants validated in DNA reference datasets, whereas false positive (FP) means the RNA variants not included in the DNA reference datasets. c Composition of RNA variant types in false positive (RNA_FP) and true positive RNA (RNA_TP) variant calls. d A T-to-C variant (located in chr17: 74866471) detected by both DNA-seq and RNA-seq is visualized in IGV. The corresponding Glu-to-Arg variant was also detected by LC-MS/MS-based proteomics