Fig. 5

Quartet DNA reference materials can be used to identify and mitigate batch effects in DNA sequencing. The scatterplots of the third and the fourth eigenvectors generated from PCA show batch effects in a SNVs, b small indels, c large deletions, and d large insertions. e Reproducibility of variants called on the whole-genome region before and after filtration. f Precision of variants called inside the benchmark regions before and after filtration. g Recall of variants called inside the benchmark regions before and after filtration