Fig. 2

Verification of structural variations by PacBio HiFi and Nanopore ultra-long reads, and Hi-C data. A Number of PacBio HiFi and Nanopore ultra-long reads crossing the regions between 2-kb upstream and downstream of the breakpoints. All Nanopore ultra-long reads are larger than 100 kb in size. B Schematic diagram of long HiFi reads crossing the breakpoints B and J. Inverted triangles point to the breakpoints. Dark colors in the bars represent repeat sequence regions. Red solid lines and red dotted lines represent a set of homologous regions between the dikaryotic genomes. Blue bars represent reads mapped and light green bars represent reads mapped by reverse complementarity, respectively. C Heat map of Hi-C data supporting neighbor relationships between upstream and downstream sequences of breakpoints B and J. Black arrowheads mark breakpoints of chromosome rearrangement