Fig. 4

Sagittal mouse brain. A The anatomy diagram of anterior and posterior mouse brains to outline the task: integrating both mouse anterior and mouse posterior to delineate the whole sagittal mouse brain. B Quantitative measurement of iLISI for eight methods (left) and SCS of derived clusters for nine methods (right), in three integration experiments: anterior1 and anterior2, posterior1 and posterior1, both anterior1/2, and posterior1/2. C Distribution of SCS of annotated domains on aligned coordinates by SPIRAL and PASTE for six-category rotated anterior replicates and posterior replicates alignments. D Spatial arrangements of Louvain clusters derived by embeddings of four methods for four samples. E Spatial distributions of raw (upper), Seurat (middle), and SPIRAL (bottom) integrated gene expressions in cortex region for anterior1 and posterior1/2. F Spatial autocorrelation of raw, Seurat, and SPIRAL-integrated domain-marker genes in four sagittal mouse brain samples. G Wilcoxon rank sum test (\(-10\mathrm{log}(\mathrm{pvalue})\)) of fold changes of domain-marker gene expressions in relative domains compared with domains having the second highest expressions