Fig. 5

Expression of TREGs in individual nuclei using smFISH with RNAscope. Representative high-magnification images showing the expression of candidate TREGs a AKT3, b ARID1B, c HK gene POLR2A, and d MALAT1 and in human DLPFC. Insets show individual nuclei with high expression (yellow arrow), low expression (green arrow), or in rare cases (≤ 14% for candidate TREGs and 22% for POLR2A, Table 1) no expression (purple arrow). Each puncta represents a single transcript, as illustrated in Fig. 1a. MALAT1 shows extremely high expression in the majority of nuclei such that individual puncta cannot be quantified (yellow arrow). Scale bar is 20 um