Fig. 4

Overview of the smFISH RNAscope experiment and DLPFC anatomy. a Illustration of RNAscope experimental design where a single DLPFC tissue block was used to generate 9 spatially adjacent slices. These 9 slices were hybridized with 3 RNAscope probe combinations noted as the AKT3, ARID1B, and MALAT1/POLR2A experiments (related to Additional file 1: Tables S4-S5). Candidate TREGs and POLR2A are shown in black, while GAD1, SLC17A7, and MBP are cell-type marker genes for inhibitory neurons (red), excitatory neurons (blue), and oligodendrocytes (orange), respectively. b Annotated image of DLPFC tissue, noting the location of gray matter (GM), white matter (WM), and sulcus. c Spatial distribution of cells expressing MBP for each sample. MBP is an oligodendrocyte cell type marker gene enriched in white matter