Fig. 2

Overview of the proportion zero filtering process. a Histogram frequency of proportion zeros for each nuclei population for a given cell type and brain region combination. These combinations are arranged with cell types along the rows [astrocytes (Astro), microglia (Micro), oligodendrocytes (Oligo), oligodendrocyte precursor cells (OPC), excitatory (Excit), and inhibitory neurons (Inhib)] and by brain region along the columns [amygdala (AMY), dorsolateral prefrontal cortex (DLPFC), hippocampus (HPC), nucleus accumbens (NAc), and subgenual anterior cingulate cortex (sACC)]. Consistent with the inhibitory neuron-rich cell type composition of the NAc, there were no excitatory neurons found in this region and therefore no data to report. The red dashed line represents the 0.75 cutoff for filtering. b Proportion zero filtering process detailed for AKT3, ARID1B, and MALAT1 compared to the classic HK gene POLR2A. If any cell type and brain region combination (individual colored points) has a proportion zero > 0.75, then the gene fails the filtering step. Unlike AKT3, ARID1B, and MALAT1, POLR2A fails proportion zero filtering