Fig. 3

PfAP2-G5 and PfAP2-G binding affinity predicted from MalariaSED are consistent with previously published ChIP-qPCR results. A PfAP2-G5 ChIP-Seq intensity along the promoter region of ap2-g during the trophozoits stage. ChIP-seq results come from a recent study [34]. The flanking region [− 100, + 100 bp] of each mutation on PfAP2-G5 binding sites is highlighted as S1 (dark red), S2 (black), and S3 (brown). B The binding affinity predicted from MalariaSED is consistent with the recently published ChIP-qPCR results [34]. The height of each bar indicates the predicted binding affinity of PfAP2-G5 in three mutants highlighted in A. C PfAP2-G ChIP-Seq intensity [6] along the promoter region of ap2-g in early sexual rings. The S4 (dark yellow) indicates flanking region [− 100, + 100 bp] of the mutation of the PfAP2-G motif. D Similar to B, but showing the predicted binding affinity of the mutant S4 in C. The results are in concordance with the published ChIP-qPCR result [6] that PfAP2-G is not able to bind in the mutant