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Table 2 Comparing different whole genome sequencing technologies

From: Genomic variant benchmark: if you cannot measure it, you cannot improve it

Technology

Company

Device name

Read length (bp)

Input DNA (microgram)

Cost

Sequencing time (hour)

Sequencing by synthesis

Illumina

NovaSeq 6000

100–300

0.3e

$

44d

Single Molecule Real-Time

PacBio

Sequel IIE

~ 15 k

5b

$$$

30c

Nanopore

ONT

PromethION

Up to 4 m

30f

$$

60

Tell-seq

Universal Sequencing Technology

TELL-Seq Library Prep Kit

100 k range information

5ngg

$i + Illumina cost

3 + Illumina run

Illumina Complete Long-Read technology (previously announced as “Infinity”)

Illumina

N/A

Up to 10 k

50ngj

$

N/A

AVITI

Element

Element AVITI System

2*150 or 10 k

0.1–0.5

$

48a

Ultima

Ultima Genomics

UG 100

300

0.25

$h

20

Sequencing by binding

PacBio (formerly Omniome)

N/A

200

N/A

N/A

N/A

  1. ahttps://miroculus.com/wp-content/uploads/2022/07/Elemet-AVITI-App-Note-03A.pdf
  2. bhttps://www.pacb.com/wp-content/uploads/Procedure-Checklist-Preparing-HiFi-SMRTbell-Libraries-using-SMRTbell-Express-Template-Prep-Kit-2.0.pdf
  3. chttps://www.pacb.com/technology/hifi-sequencing/sequel-system/
  4. dhttps://emea.illumina.com/systems/sequencing-platforms/novaseq.html
  5. ehttps://www.illumina.com/content/dam/illumina/gcs/assembled-assets/marketing-literature/illumina-dna-pcr-free-loading-concentration-tech-note-770-2020-007/illumina-dna-pcr-free-loading-concentration-tech-note-770-2020-007.pdf
  6. fhttps://nanoporetech.com/sites/default/files/s3/literature/PromethION-brochure.pdf
  7. ghttps://sagescience.com/wp-content/uploads/2020/03/TELL-Seq_AGBT_2020_print.pdf
  8. hhttps://www.science.org/content/article/100-genome-new-dna-sequencers-could-be-game-changer-biology-medicine
  9. i900$ for the kit https://www.universalsequencing.com/shop
  10. jhttps://www.illumina.com/products/by-brand/complete-long-reads-portfolio.html