Fig. 5

The association between Dynamic GbM and regulatory chromatin marks. A Boxplots showing the normalized read depth of ChIP-seq data of fourteen histone modifications (data from [43]) within exons overlapping Dynamic GbM domains (blue), Stable GbM domains (green), or total exons (gray). Horizontal lines = median, box = interquartile range, whiskers = 5th and 95th percentiles. Statistical test for effect size = Cohen’s D. B The percentage overlap between Stable and Dynamic GbM gene bodies and four chromatin states identified by [45]. C The total base pair overlap between Stable and Dynamic GbM gene bodies and a chromatin state typically enriched in distal promoters. D Boxplot showing the distribution of gene body lengths of both Stable and Dynamic GbM genes. E Boxplot showing the normalized read depth of H3K4me2 ChIP-seq data at Stable and Dynamic GbM genes separated into bins based on gene body length. For C,D,E, *p =  < 0.0001 (two-tailed parametric t-test). F Genome browser snapshot of raw H3K4me1 and H3K4me2 ChIP-seq data at Stable and Dynamic GbM genes. Tracks represent WT data from four independent studies. Data obtained from [46]. G Boxplots showing the cellular heterogeneity of CGs within the methylated domains of Dynamic GbM genes in WT and two mutant backgrounds with reduced H3K4me2, atx1;2;4;5 and sdg2. *p =  < 0.0001 (non-parametric two-tailed t-test)