Fig. 1

Two distinct types of gene body methylation (GbM) in the Arabidopsis genome. A Genome browser snapshot showing representative example of a gene with Stable GbM and a gene with Dynamic GbM in both WT and drdd mutants. Red bars represent methylated cytosines, and the height of the bar represents the percentage of cells in which a cytosine is methylated (scale = 0–100%). B Venn diagram showing the number of Stable and Dynamic GbM genes and the overlap between the two groups. C Box plot showing the overall percentage of CG methylation across entire genes (all CGs across all cells) with Stable or Dynamic GbM. Line = median, box = interquartile range, whiskers = 5th and 95th percentile. *p =  < 0.0001 (two-tailed t-test). D Violin plot showing the distribution of methylation heterogeneity levels of all CGs in the Arabidopsis genome, shown as the percentage of cells in which a CG is methylated. E The proportion of fully methylated, heterogeneously methylated, and unmethylated CGs within both Stable and Dynamic GbM gene bodies, in both WT and drdd mutants