Fig. 5

Data preparation for evaluating demultiplexing and the pipeline of HycDemux. A DNA sequence after multi-sample sequencing library construction. B Extract “barcode” sequences (nanopore signals) within sequences (nanopore signals) using a fast heuristic scheme (see the "Materials and methods" section). C Complete pipeline of HycDemux for demultiplexing consists of several steps. First, the data preparation pipeline completes the necessary preprocessing of the data. Next, the hybrid clustering algorithm (Fig. 1A, B, C) performs the data clustering process. Finally, the demultiplexing module (Fig. 1D) completes the final step of demultiplexing