Fig. 3

Effects of the 108-bp insertion in the exon of the SPN gene in Hainan cattle on Mycobacterium tuberculosis. A Schematic representation showing the chromosomal position of the 108-bp insertion and the partial sequences of the 108-bp insertion obtained by genome alignment. B Schematic representation of the CD43 protein structures of taurine (WT-SPN) and Hainan (HN-SPN) cattle. C, D The RAW264.7 cells were transfected with pCMV-WT-SPN or pCMV-HN-SPN for 48 h and then treated with the H37Ra strain (MOI = 0, 5, 10, 20, 30, and 50) for 4 h. The H37Ra strain was detected by immunofluorescence. E The RAW264.7 cells were transfected with pCMV-WT-SPN or pCMV-HN-SPN for 24 h and then treated with the H37Ra strain (MOI = 10) for 24 h. F The mRNA levels of IL-12, IL-10, IL-6, and TNFα were determined using qRT-PCR