Fig. 4

Splicing efficiency and 3’ end Pol II pause mutually promote their correlations with exon numbers. a, b Scatter plot indicating the correlation between 3’PI and 3’SS ratio (left) or 5’SS ratio (right). Only the genes with both SS ratio and 3’PI are included. The Spearman correlation coefficient was shown above the plot. The orange line indicates the trendline fitted based on the least squares method. c Box plots demonstrate the relationship between 3’PI and exon numbers in gene groups with different levels of 3’SS ratio. The plot in blue demonstrates the situation of all protein-coding genes. Protein-coding genes were divided into five groups according to average 3’SS ratios of genes (part 0 to part 4, from high to low), and the relationship between 3’PI and exon numbers was plotted separately for each group. d Box plots demonstrate the relationship between the 3’SS ratio and exon numbers in gene groups with different levels of 3’PI. The plot in blue demonstrates the situation of all protein-coding genes. Protein-coding genes were divided into five groups according to the 3’PI (part 0 to part 4, from high to low), and the relationship between the 3’SS ratio and exon numbers was plotted separately for each group. For c and d, The Spearman correlation coefficient is indicated above each plot. Gene numbers of each group were shown below the boxes