Fig. 7

ALKBH1 is required for stress-induced gene expression and is involved in plant tolerance to stress. a RT-PCR analysis of ALKBH1 target genes expression in plants treated with salt (green) and high temperature (red). Three biological replicates were performed. Student’s t-test was used to detect significance. Significance was calculated by comparing the mutant with the wild type and marked on the mutant column. b Recovery phenotypes of wild type and the transgenic plants from salt stress. The seedlings of Nipponbare (NIP, the wild type control with alkbh1 and OEALKBH1), alkbh1 and OEALKBH1 grown under 12-h light/12-h dark conditions for 14 days, transferred to 180 mM NaCl for 3 days and recovered for 7 days, respectively. Scale bar, 5 cm. Data are presented as mean ± SD (n > 3 biological replicates, and 35 plants were tested in each of the biological replicates). Significance of differences (***P < 0.001) was determined by Student’s t-test. Significance was calculated by comparing the mutant or overexpression with the wild type and marked on the mutant and overexpression column. c Leaf phenotypes of 8-week-old WT, alkbh1 and OEALKBH1 plants inoculated with the Xoo isolate PXO99A. Lesion lengths were measured and the values are means ± SEM (n > 30 biological replicates). Significance was calculated by comparing the mutant or overexpression with the wild type and marked on the mutant and overexpression column. d Phenotype and seed setting rate of the mutant and overexpressed plants under normal and high-temperature conditions. Data are presented as mean ± SD (n > 20 biological replicates). Significance of difference (***P < 0.001 was tested by Student’s t-test). Significance was calculated by comparing the mutant or overexpression with the wild type and marked on the mutant and overexpression column