Fig. 5

ALKBH1-binding inhibits H3K27me3 and promotes the expression of target genes. a Integrative Genomics Viewer of 7 stress-related transcription factor genes targeted by ALKBH1. b Three regions (red horizontal bars) of the 7 genes tested by ChIP-PCR analysis with H3K27me3 and ALKBH1 antibodies. c RT-PCR analysis of the 7 genes in wild-type and alkbh1 mutant lines. P2 primers were used. d Anti-ALKBH1 ChIP-PCR detection of ALKBH1 association with the 7 genes in wild-type and alkbh1 plants. e ChIP-PCR detection of H3K27me3 levels in the 7 genes in wild-type and alkbh1 plants. Values are the means of three biological replicates, and error bars represent ± SD. Statistical significance between wild type and alkbh1 was calculated by Student’s t-test. *P < 0.05, **P < 0.01, ***P < 0.001. n, no significant difference