Fig. 2

ALKBH1 activates genes marked by both H3K27me3 and H3K4me3 but depleted of mCG in the body region. a RNA-seq analysis of differentially expressed genes (DEGs) (> 2-fold; q-value < 0.05) in alkbh1 compared with the wild type (WT). b GO enrichment of the downregulated DEGs in alkbh1. c Metaplots and heatmaps of chromatin modification profiles in the alkbh1 downregulated (n = 1047 blue) and upregulated (n = 386 red) genes and control genes with similar transcription levels (CK) (n = 1500 gray) in the wild-type background. Expression levels of the DEGs in the mutant and the control genes in wild-type plants are shown by boxplots and heatmaps. d RNA-seq analysis of DEGs (> 2-fold; q-value < 0.05) in the overexpression lines (OEALKBH1) compared with the wild type (WT). e GO enrichment of upregulated DEGs in OEALKBH1. f Metaplots and heatmaps of chromatin modification profiles in the OEALKBH1 downregulated (n = 1114 blue) and upregulated (n = 2388 red) genes and control genes (n = 1500 gray) in the wild-type background