Table 4 Both Ariadne and reference deconvolution increase the summed NA50 of de novo assembled metagenomes. The second-to-rightmost column shows the difference between the summed NA50 of assemblies obtained from deconvolved reads and the summed NA50 of non-deconvolved assembly. The asterisk (*) indicates that the assembly did not have sufficient sequence material that was alignable to the reference sequence for an NA50 to be calculated

MOCK5 10x

Enterobacter cloacae*

Ariadne

16 655

5 598 796

MOCK20 TELL-Seq

Streptococcus agalactiae*

Ariadne

464 957

2 159 783

MOCK20 TELL-Seq

Staphylococcus epidermidis*

Ariadne

489 177

2 575 951

MOCK20 TELL-Seq

Streptococcus mutans*

Ariadne

906 518

2 031 444

MOCK20 TELL-Seq

Staphylococcus aureus*

Ariadne

106 598

2 915 427

MOCK5 10x

Enterobacter cloacae*

Reference

13 628

5 598 796

MOCK20 10x

Pseudomonas aeruginosa

Reference

5 477 921

6 374 461

MOCK20 10x

Rhodobacter sphaeroides

Reference

-580 531

4 366 774

MOCK20 10x

Staphylococcus epidermidis

Reference

639 974

2 575 951

MOCK20 TELL-Seq

Bacillus cereus

Reference

1 551 522

5 442 819

MOCK20 TELL-Seq

Staphylococcus epidermidis*

Reference

1 503 747

2 575 951

MOCK20 TELL-Seq

Streptococcus agalactiae*

Reference

2 047 925

2 159 783

MOCK20 TELL-Seq

Streptococcus mutans*

Reference

2 012 488

2 031 444

MOCK20 TELL-Seq

Staphylococcus aureus*

Reference

134 663

2 915 427