Fig. 3

Halving and doubling the maximum fragment length does not meaningfully change the quality of de novo assembly using reference-deconvolved linked-reads. Shown here are the NA50, largest alignments, and relative misassembly rate of the MOCK5 LoopSeq reference-deconvolved assembly. As before, we compared assemblies built from raw linked reads (no deconvolution) to assemblies built from reads deconvolved using two methods: reference deconvolution with maximum fragment lengths set to 100 kbp (Ref_100), 200 kbp (Ref_200), and 400 kbp (Ref_400), and deconvolution using Ariadne