Fig. 9

Phenotype of GhLAX1, GhLAX2, GhLOX3 knock out and overexpression callus with hypocotyls as explants. a Schematic view of gRNA1, gRNA2 target sites in the GhLAX1, and GhLOX3 and overexpression cassette of GhLAX2. b Paraffin sections of hypocotyls infected with Agrobacterium after induction on callus induction medium for 0, 24, and 72 h. The red box represents the proliferation site. c The phenotypes of different transgenic explants and control (P7N) at 20 days post-induction and the callus proliferation rate (CPR) of explants and control at 20 days post-induction. d The phenotype of callus on the GhLAX1 knock out and GhLAX2 overexpression explants at about 70 days post-induction. Scale bar, 100 μm. e Days of embryonic callus occurrence of different transgenic explants. f Morphology of somatic cell embryos of JOE1. Scale bar, 100 μm