Fig. 5

Pseudotime trajectory of primary vascular cells. a, b Pseudotime analysis of primary xylem, xylem vessel precursors, parenchyma, and cambium cells of Jin668 and TM-1. Each dot indicates a single cell, and the color of the upper right corner represents the starting point and end point of differentiation. c, d Expression patterns of representative auxin-related genes (WAT1 and IAA4) are shown over the course of pseudo-time. Color bar indicates the relative expression level. e Heatmap showing the expression of the branch-dependent genes over pseudo time. GO terms of SE-related are shown in the table on the right. The middle of the heatmap is the beginning of pseudo time. Both sides of the heatmap are the end of pseudo time. Color bar indicates the relative expression level. J(T)PX, primary xylem of Jin668 (TM-1); J(T)XV, xylem vessel precursors of Jin668 (TM-1); J(T)PC, parenchyma and cambium region of Jin668 (TM-1)